2021年4月19日 (一) 21:14的版本

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Carbon Dioxide Capturer 3.0

Presented by Anhui University of Technology (AHUT) and Zhejiang University (ZJU).

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Carbon dioxide (CO₂) mainly from the combustion of fossil fuels is primary greenhouse gas associated with global warming and climate change; therefore, reducing the amount of CO₂ emitted into the atmosphere is an urgent need, of which CO₂ capture has become an extensively investigated area of research. In this project, we decided to use the biomimetic method of the enzyme (carbonic anhydrase, CA) for capturing CO₂, which has the advantages of safety, high catalytic efficiency and environment-friendly compared with other methods. We designed an efficient and stable CA by improving the catalytic performance of CA from the OT3 strain of the hyperthermophilic archeon Pyrococcus horikoshii for capturing CO₂. We use computer-aided analysis software to predict the ideal mutation site of the OT3-CA for improving its catalytic activity. In conclusion, we constructed a new part OT3-CA-WT and obtained its protein with higher thermostability, which is able to maintain its activity at 45 °C and 70°C. Furthermore, we obtained the mutant OT3-CA via molecular simulation with enhanced catalytic activity. The thermostability and efficiency of OT3-CA make it a potential candidate for CO₂ capture.

"

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Carbonic anhydrase is a zinc metalloenzyme that catalyzes the reversible reaction of CO₂ and H₂O to form HCO3^- and H⁺. It is widely found in animals, plants and microorganisms, and plays an important role in biological processes such as carbon dioxide transfer, photosynthesis, acid-base balance and ion transport in the body.

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The first step in the reaction is for the HYDROXyl group to act as a nucleophile and attack the carbon atoms in the CO₂ molecule. In the presence of water molecules, hydroxyl ions and CO₂ complexes are replaced by water molecules, detach from the active centers, and form bicarbonate ions. The second step is the regeneration of the active center Zn²⁺ -OH ^-. When certain amino acids are used as hydrogen ion transport carriers, water molecules spontaneously ionize and dehydrogenate, and hydrogen ions are transported out of the hydrophobic center. The proton transport rate of the active center is the rate-limiting step of the enzyme-catalyzed reaction. The K_at value of most carbonic anhydrases is >10⁶ s^-1.

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Widget:新样式：修订间差异

2021年4月19日 (一) 21:14的版本

Carbon Dioxide Capturer 3.0

Presented by Anhui University of Technology (AHUT) and Zhejiang University (ZJU).

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2021年4月19日 (一) 21:14的版本

Carbon Dioxide Capturer 3.0

Presented by Anhui University of Technology (AHUT) and Zhejiang University (ZJU).

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